Biological activities of phenolic compounds extracted from flaxseed meal

Engy M Akl,Fakhriya S Taha,Samira S Mohamed,Ahmed I Hashem

doi:10.1186/s42269-020-0280-x

Abstract

BackgroundThere is a worldwide demand for phenolic compounds (PC) because they exhibit several biological activities. The present investigation deals with a comprehensive study on the biological activities of phenolic compounds extracted from flaxseed meal (FM) with the aid of ultrasonic waves.ResultsThe antioxidant activity of the PC extract of FM is considerably high when measuring it by the three methods (the β-carotene coupled oxidation method, the DPPH free radical scavenging activity method, and measuring the reducing antioxidant power). The toxicity test revealed that the PC extract was nontoxic on normal retina cell line. Also, it has no anticoagulating activity. Evaluation of antimicrobial activity showed that it is effective towards four strains only from seven. FM phenolic extract has been evaluated as chemo-preventive agents by testing the product for any cytotoxic activity against human tumor cell lines. The highest inhibitory effect was achieved on cell lines of colon carcinoma and lung carcinoma with IC50 = 22.3 and 22.6 μ/ml respectively.ConclusionThe PC extracted from FM showed high antioxidant activity, nontoxic on normal retina cell line, no anticoagulating activities, and an antimicrobial effect on some pathogenic bacteria, so the phenolic compounds extracted from flaxseed meal showed significant biological activities.

Highlights

The components of flaxseed meal were found to have a wide spectrum of biological activity
The antioxidant activity of phenolic compounds (PC) extract (Table 1) prepared by Ultrasonic-assisted extraction (UAE) from flaxseed meal (FM) was determined by three methods: (1) β-carotene coupled oxidation method, (2) DPPH free radical scavenging activity method, and (3) the reducing antioxidant power
Nil carcinoma cell line (HTC), lung carcinoma sell line (A549), liver carcinoma cell line (HEPG2) FM phenolic extract had no effect on normal fibroblasts

Summary

Introduction

The components of flaxseed meal were found to have a wide spectrum of biological activity. Ground/milled and whole flaxseeds stored showed negligible oxidative deterioration at room temperature, which indicates the presence of an efficient antioxidant system, where compounds other than tocopherols may play an important function [Chen et al 1994); Malcolmson et al (2000); Hosseinian et al (2006)]. Flaxseed is rich in lignans, e.g., secoisolariciresinol diglucoside (SDG), which are present in flaxseed oil. These components have some antioxidant activity (Hosseinian et al 2006). The present investigation deals with a comprehensive study on the biological activities of phenolic compounds extracted from flaxseed meal (FM) with the aid of ultrasonic waves

Methods

Results

Discussion

Conclusion