Biological activities of green synthesis silver nanoparticles by Plantago lanceolata L. leaves

Abstract

In our study, silver nanoparticle (AgNP) was synthesized by green synthesis method using Plantago lanceolata L. leaves. The synthesized AgNPs were characterized using energy-dispersive spectra (EDS), scanning electron microscopy (SEM) and transmission electron microscope (TEM). The AgNPs were with an average size of 10–25 nm and mostly spherical. The antimicrobial potential of AgNPs was tested against some bacteria and a yeast culture by disc diffusion and minimum inhibitory concentration (MIC) methods. The AgNPs were significantly inhibited all test culture except Bacillus subtilis ATCC 6633. Antioxidant activities were determined by 2,2-diphenyl-1-picrylhydrazil (DPPH) free radical scavenging and 2,2'- Azino-bis (3-ethylbenzothiazolin-6-sulfonic acid) (ABTS•+) cation radical scavenging activity. The higher DPPH and ABTS of AgNPs were determined 33.20±0.50% and 39.37±0.54%, respectively. Thus, green synthesis AgNPs may be a new alternative therapeutic agent for infection therapy.