Abstract
Human Fortilin, an antiapoptotic protein, has also been implicated in several diseases; however, several potential uses of fortilin have also been proposed. Bearing the implications of fortilin in mind, fortilin analog, which has no complication with diseases, is required. Since a recombinant full-length fortilin from Fenneropenaeus merguiensis (rFm-Fortilin (FL)) reported only 44% (3e-27) homologous to human fortilin, therefore the biological activities of the Fm-Fortilin (FL) and its fragments (F2, F12, and F23) were investigated for potential use against HEMA toxicity from filling cement to pulp cell. The rFm-Fortilin FL, F2, 12, and F23 were expressed and assayed for proliferation activity. The rFm-Fortilin (FL) showed proliferation activity on human dental pulp cells (HDPCs) and protected the cells from 2-hydroxy-ethyl methacrylate (HEMA) at 1–20 ng/ml. In contrast, none of the rFm-Fortilin fragments promoted HDPC growth that may be due to a lack of three conserved amino acid residues together for binding with the surface of Rab GTPase for proliferative activity. In addition, rFm-Fortilin (FL) activated mineralization and trend to suppressed production of proinflammatory cytokines, including histamine (at 10 ng/ml) and TNF-α (at 100 ng/ml). Besides, the rFm-Fortilin (FL) did not mutate the Chinese hamster ovary (CHO) cell. Therefore, the rFm-Fortilin (FL) has the potential use as a supplementary medical material to promote cell proliferation in patients suffering severe tooth decay and other conditions.
Highlights
Controlled tumor protein (TCTP) is an antiapoptotic protein, known as fortilin, which has 172 amino acids and its sequence is significantly conserved among eukaryotes; the majority of fortilin is found in the nucleus of the cell [1, 2]
Cells treated with rFm-Fortilin (FL) at both 24 and 72 h showed the same pattern of increasing percentages of viable cells with increased rFm-Fortilin (FL) concentrations
The results of this study confirmed that rFm-Fortilin could promote human dental pulp cells (HDPCs) proliferation, which is consistent with the rFm-glutathione S-transferase (GST)-Translationally controlled tumor protein (TCTP) in previous studies [9]
Summary
Controlled tumor protein (TCTP) is an antiapoptotic protein, known as fortilin, which has 172 amino acids and its sequence is significantly conserved among eukaryotes; the majority of fortilin is found in the nucleus of the cell [1, 2]. Fortilin does not have a structure similar to inhibitors of apoptosis proteins or the Bcl family, fortilin has been proposed to control cell apoptosis [2]. Activities of a recombinant fortilin from Fenneropenaeus merguiensis
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