Biological activities and characterization of polysaccharides from the three Australian Sweet Lupins

Solomon Rajesh Thambiraj,Narsimha Reddy,Michael Phillips,Sundar Rao Koyyalamudi

doi:10.1080/10942912.2019.1588298

Solomon Rajesh Thambiraj, Narsimha Reddy + Show 2 more

Open Access

https://doi.org/10.1080/10942912.2019.1588298

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Abstract

ABSTRACT Lupin is a major rotational crop produced abundantly in Australia. Three varieties of sweet lupins are grown in Australia depending on soil types and climatic conditions; these are white lupin (Lupinus albus), blue lupin (Lupinus angustifolius), and yellow lupin (Lupinus luteus). In this study, polysaccharides from the three lupin species have been extracted with hot water and a range of their biological activities investigated. The antioxidant activities of lupin polysaccharides have been measured in terms of ABTS•+ radical and hydroxyl radical scavenging activities, and their affinity to chelate with iron. Immune-enhancing power of the lupin polysaccharides was examined by measuring their capacity to stimulate the macrophages (RAW 264.7) to release TNF-α and nitric oxide. The prebiotic activities were determined against the beneficial probiotics such as Lactobacillus rhamnosus DR20, Lactobacillus acidophilus LAFTI L10, Bifidobacterium lactis DR10, and Bifidobacterium animalis BB-12. Polysaccharides from the three lupin species have exhibited significant antioxidant, immunostimulatory, and prebiotic activities. The results demonstrated that blue lupins displayed the best activities, and therefore are expected to possess high potential to be used as nutraceutical and functional ingredients with significant therapeutic value. FT-IR spectroscopic technique was employed for a preliminary structural characterization. Studies involving surface morphology and thermal behavior have indicated that the lupin polysaccharides are suitable for end product development.

Highlights

Lupin is a major crop cultivated by Australian farmers to fix soil nitrogen in wheat and other cereal producing fields.[1]
The polysaccharides extracted from blue, white and yellow lupins are designated as Blue lupin (BLP), White lupin (WLP), and Yellow lupin (YLP), respectively
Whereas WLPs have produced about fourfold increase and YLPs have increased threefold. These results indicate that the BLPs are the most active amongst the three lupin species with respect to TNF-α production, and the yellow lupin polysaccharides (YLPs) displayed lowest activity

Summary

Introduction

Lupin is a major crop cultivated by Australian farmers to fix soil nitrogen in wheat and other cereal producing fields.[1]. Lupin offers a suitable option for sustainable use of land and is of immense value to Australian farmers. Further research to improve commercial value of the grain will be beneficial to farmers and food/nutraceutical industry as well as the consumers. Investigations of bioactivities of the major constituents from the three sweet lupins are likely to reveal the nutraceutical/pharmaceutical benefits and will improve the value of lupin seeds. Literature demonstrates[5,7,8] that lupin has large amounts of beneficial polysaccharides and protein. These two nutrients can be isolated from lupin and can be utilized for nutraceutical/pharmaceutical purposes

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