Abstract
Quantification of specific antibodies underpins the assessment of adaptive immunity in response to vaccination or infection and is performed by enzyme-linked immunosorbent assay (ELISA). A biolayer interferometry (BLI) assay was recently developed that simultaneously quantifies IgM antibodies and their avidity in giant grouper (Epinephelus lanceolatus) sera and proved to be a robust, repeatable and more high-throughput alternative to ELISA [1]. Here we attempted to optimise a similar single-step BLI assay using an Octet HTX instrument to quantify IgM specific to the hapten 2,4-dinitrophenol (DNP) in serum from Atlantic salmon (Salmo salar) primed and boosted with DNP conjugated to keyhole limpet hemocyanin.
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