Biointerface topography mediates the interplay between endothelial cells and monocytes.

Yan Liu,Wenshuai Deng,Zhibin Wang,Patrick Van Rijn,Tao Yu,Liangliang Yang,Qihui Zhou,Xiuxiu Fu

doi:10.1039/d0ra00704h

Abstract

Endothelial cell (EC) monolayers located in the inner lining of blood vessels serve as a semipermeable barrier between circulating blood and surrounding tissues. The structure and function of the EC monolayer affect the recruitment and adhesion of monocytes, which plays a pivotal role in the development of inflammation and atherosclerosis. Here we investigate the effect of material wrinkled topographies on the responses of human umbilical vein endothelial cells (HUVECs) and adhesion of monocytes to HUVECs. It is found that HUVEC responses are non-linearly mediated by surface topographies with different dimensions. Specifically, more cell elongation and better cell orientation on the wrinkled surface with a 3.5 μm amplitude and 10 μm wavelength (W10) are observed compared to other surfaces. The proliferation rate of HUVECs on the W10 surface is higher than that on other surfaces due to more 5-ethynyl-2′-deoxyuridine (EdU) detected on the W10 surface. Also, greater expression of inflammatory cytokines from HUVECs and adhesion of monocytes to HUVECs on the W10 surface is shown than other surfaces due to greater expression of p-AKT and ICAM, respectively. This study offers a new in vitro system to understand the interplay between HUVEC monolayers and monocytes mediated by aligned topographies, which may be useful for vascular repair and disease modeling for drug testing.

Highlights

The transport mediated through blood vessels plays a critical role in in ammation, barrier formation, wound healing, as well as tissue morphogenesis and function.[1]
A er imprinting on the wrinkled PDMS samples as illustrated in Table 1, wrinkled topographies with various dimensions (i.e., W0.5A0.05, W3A0.7, W10A3.5 and W27A4.3) were obtained (The reference submitted to Biomaterials Science), which were designated as W0.5, W3, W10, and W27, respectively
It was found that human umbilical vein endothelial cells (HUVECs) responses were non-linearly mediated by surface topographies

Summary

Introduction

The transport mediated through blood vessels plays a critical role in in ammation, barrier formation, wound healing, as well as tissue morphogenesis and function.[1] Endothelial cells (ECs) form a continuous inner monolayer lining of blood vessels, and serve as a semipermeable barrier between circulating blood and surrounding tissues as well as the key regulator of in ammation and vascular homeostasis.[2,3] The adhesion of circulating monocytes to ECs is pivotal for the development of in ammation and atherosclerosis.[4,5] Later, the attached monocytes migrate into the intima, differentiate into macrophages by macrophage colony-stimulating factor (M-CSF),[6,7,8] and regulate vascular remodeling.[9] it is vital to elucidate how to control the interaction between ECs and monocytes. Elucidating how directional topography mediates the interplay between ECs and monocytes plays a critical role in improving the development of the vascular gra material

Methods

Results

Conclusion