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Abstract
Objective: To investigate the bioinformatics of differentially expressed proteins in plasma in patients with acute cerebral infarction associated with H-type hypertension. Methods: Gene chip public database (gene expression omnibus, GEO) GDS4521 chip data, in the chip in 30 cases of H patients with acute cerebral infarction with high blood pressure and age, gender, matching the 20 H hypertension patients as the research object, collects the mononuclear cells (PBMCs) is used to detect the gene chip, using the GO (gene Ontology, GO), protein function analysis tools such as KEGG, screening and analysis of enrichment of differentially expressed genes function and related signaling pathway. Results: 31 genes in PBMCs were significantly changed in h-type hypertension and H-type hypertension with acute cerebral infarction, 32 of which were increased and 2 of which were decreased. GO analysis showed that in terms of biological processes, the genes related to inflammatory response and neutrophil chemotaxis were the most. In terms of molecular function, chemokine activity-related genes are the most. KEGG signaling pathway analysis showed that the most differentially expressed genes were located in the TNF signaling pathway. Conclusion: H-type hypertension with acute cerebral infarction has a variety of functional proteins and signaling pathways changes, suggesting that inflammatory response in H-type hypertension with acute cerebral infarction recovery period may still play a role in the prognosis and reactivation of the disease.
Highlights
Cerebrovascular disease has become the first cause of disability and death among urban and rural population in China, and its incidence tends to increase year by year
H-type hypertension with acute cerebral infarction has a variety of functional proteins and signaling pathways changes, suggesting that inflammatory response in H-type hypertension with acute cerebral infarction recovery period may still play a role in the prognosis and reactivation of the disease
GO, KEGG, DAVID, G: Profiler and other online analysis tools are commonly used in bioinformatics analysis, which can carry out gene ontology GO enrichment analysis, KEGG signal pathway analysis, functional annotation and so on for a large number of genes
Summary
Cerebrovascular disease has become the first cause of disability and death among urban and rural population in China, and its incidence tends to increase year by year. Proteomics is a large-scale study of protein expression levels, post-translational modifications, and protein-protein interactions [12] It can dynamically, comprehensively and quantitatively investigate the changes of protein types and quantities in the process of disease development, and can complete the extremely complex research on protein function and cytokine changes. To identify the two differential proteins at the same time to distinguish them from etiological targets It will bring a broad prospect for the etiology, early warning, diagnosis and treatment, new methods and techniques for prognosis and the establishment of an assessment and analysis system for this disease. The differential protein of emergency expression in the early stage of acute cerebral infarction was identified to distinguish from the etiological target. To promote the disciplinary development of this field through substantive interdisciplinary research
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