Bioinformatics Study of Osteoclastogenesis Inhibitory Factor and Receptor Activator of NF-κB Ligand Expression in Ovariectomized Osteoporosis Rat Model Using Probiotics Preparation

Abstract

Objective: To study the expression of OPG and RANKL in the ovariectomized osteoporosis SD rat model by probiotics preparation, and to observe the mechanism of probiotics regulating bone metabolism by medical imaging, and finally improve and improve the bone mass level of the elderly. Methods: Forty five 12 weeks old SD female rats were randomly were evenly divided into control group (Sham operation, Sham), ovariotomy group (Ovariectomy, the OVX), ovarian resection and probiotic preparations intervention group (OVX + Probiotics), each group of 15, in addition to the Sham group ovaries removed around the same size of adipose tissue, the OVX + Probiotics group and the OVX rats were performed bilateral ovaries removed. Eight weeks after the surgery, each group randomly, 2 rats dual-energy X-ray absorptiometry measurement, determine after the success of the building, the OVX + Probiotics group feed gavage quad living bacterium equivalent dose concentration solution, Sham group and the OVX group was given distilled water filling and feed volume. After 4 weeks of continuous administration, all rats were sacrificed. Subsequently, Tb. N, Tb. Th, Tb. Sp and BV/TV were measured by micro-CT of medical imaging technique. The levels of serum BGP, ALP and Ca were measured by automatic biochemical analyzer. Real-time quantitative PCR and the expression levels of OPG, β-actin and RANKL genes and proteins were detected by Western blot. Results: Compared with the Sham group, compared with the, the OVX + Probiotics group and the OVX rats femur lower Tb. N, Tb. Th, BV/TV value decreased significantly, Tb. Sp increased significantly, serum BGP and ALP, Ca levels were significantly lower (P < 0.05), the OVX + Probiotics group compared with OVX group, the rat femur lower Tb. N, Tb. Th, BV/TV value, Tb. Sp, reducing RANKL protein expression and serum BGP and ALP, Ca levels, OPG protein expression were higher. The difference was statistically significant (P < 0 05), after 4 weeks intragastric administration. Conclusion: The probiotic preparation (Bifidobacterium quadruple live bacteria) has an effect on the OPG/RANKL/RANK axis system, which plays an anti-OP role, and provides a new research direction for the prevention and treatment of postmenopausal osteoporosis.