Bioinformatics prediction of miR-30a targets and its inhibition of cell proliferation of osteosarcoma by up-regulating the expression of PTEN

Biao Zhong,Yukai Wang,Shang Guo,Changqing Zhang,Chi Zhang,Wei Zhang

doi:10.1186/s12920-017-0300-3

Biao Zhong, Yukai Wang + Show 4 more

Open Access

https://doi.org/10.1186/s12920-017-0300-3

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Abstract

BackgroundMiRNAs are frequently abnormally expressed in the progression of human osteosarcoma. Phosphatase and tensin homologue deleted on chromosome 10 (PTEN) is one of the tumor suppressors in various types of human cancer. In the present study, we detected how hsa-miR-30a-3p regulated PTEN and further tested the role of hsa-miR-30a-3p in the cell proliferation of osteosarcoma cells.MethodsThe levels of miR-30a were determined by real time PCR. The expression of PTEN was tested by western blotting analysis. Cell distribution of PTEN was observed with confocal laser scanning microscope. Cell viability was determined by MTT assay.ResultsThe expression of miR-30a and PTEN was obviously decreased in MG-63, 143B and Saos-2 cells compared with primary osteoblasts. TargetScan analysis data showed miR-30a might bind with position 30-57 of 3’UTR of PTEN. Transfection with miR-30a-3p increased the level of PTEN in MG-63 cells, while transfection with miR-30a-3p inhibitor significantly decreased the expression of PTEN in osteosarcoma cells. Transfection with miR-30a-3p significantly inhibited cell proliferation of osteosarcoma cells, while miR-30a inhibitor obviously promoted cell viability of MG63 cells and Saos-2 cells. Inhibition of PTEN eliminated the proliferation inhibitory effect of miR-30a-3p.ConclusionThus, all these findings revealed the anti-tumor effects of miR-30a in human osteosarcoma cells, which could be mediated by regulating the level of PTEN.

Highlights

MiRNAs are frequently abnormally expressed in the progression of human osteosarcoma
The levels of PTEN were detected by western blotting analysis and the results demonstrated that the expression of PTEN was obviously decreased in MG63, 143B and Saos-2 cell line compared with that in primary osteoblasts (Fig. 1a)
In order to test the role of miR-30a in human osteosarcoma cell lines, MG63 cells were transfected with miR-30a inhibitor and inhibitor negative control and cultured for 24 h

Summary

Introduction

MiRNAs are frequently abnormally expressed in the progression of human osteosarcoma. Phosphatase and tensin homologue deleted on chromosome 10 (PTEN) is one of the tumor suppressors in various types of human cancer. We detected how hsa-miR-30a-3p regulated PTEN and further tested the role of hsa-miR30a-3p in the cell proliferation of osteosarcoma cells. The biological functions of most miRNAs are not yet fully understood, they may have a key role in the regulation of various biological processes [1]. The levels of miR-106b were significantly higher in osteosarcoma, which functioned as an oncogene to promote the progression of osteosarcoma [6]. MiR-1247 was detected to work as a potential tumor suppressor by targeting MAP3K9 in progression of osteosarcoma [7]

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