Bioinformatics of erythroblast transformation-specific related gene and protein

Abstract

Objective To investigate the function and regulatory mechanisms of human erythroblast transformation-specific (ETS) related gene (ERG) in human disease. Methods ERG gene(NP_001230357) and protein sequences (NP_001230357) were screened from GenBank. The differences in different species, subcelluar location, secondary structure, functional domains and interaction networks were analyzed by using bioinformatic tools. Results The ERG gene encoded a length of 468 amino acid protein with two functional domains: PNT and ETS. Its molecular weight was 53 837.51, theoretical isoelectric point was 7.29. α-helix secondary structure (H) accounted for 12.35%, β fold (E) for 3.50%, and random coil for 84.16%. ERG protein contained four potential N-linked glycosylation sites, 11 potential casein kinase II phosphorylation sites, five cardamom acylation sites, seven protein kinase C (PKC) phosphorylation sites and one tyrosine kinase phosphorylation site. Its interaction networks were further analyzed by STRING. Conclusion More biological information about ERG gene and protein was analyzed, which provides some basic information for further research. Key words: Transmembrane-serineprotease gene 2-erythroblast transformation-specific related gene; Prostate cancer; Bioinformatics