Abstract
WRKY transcription factors are very important transcriptional regulators in the process of plant response to plant growth and abiotic stresses. In this study, the open reading frame (ORF) of soybean GmWRKY32 gene was cloned from the WRKY sequence of soybean genome, which was a polypeptide encoding 300 amino acids. Bioinformatics analysis revealed that the WRKY32 protein contained a highly conserved WRKY domain and belonged to Group III of the WRKY gene family. The homology with Glycine soja GsWRKY70, Cajanus cajan CcWRKY70 and Vigna angularis VaWRKY70 proteins was 85%, 79% and 72%, respectively. In addition, GmWRKY32 , is an insoluble protein, no signal peptide and transmembrane domain. Further analysis of the gene expression pattern under different stress and hormone induction showed that after 12 h of treatment with PEG, high salt stress and JA, the expression of GmWRKY32 was increased by 4.5, 4.2 and 3.2 times than that of control, respectively. After 24 h of ABA induction, the expression level of GmWRKY32 was increased 6-fold compared with that of the control. Conjecture that it is closely related to the resistance of soybean to abiotic stress which would lay a foundation for the family structure and functional research of soybean WRKY transcription factors, and will provide scientific basis for research of soybean resistance and molecular breeding.
Highlights
Drought stress is one of the important environmental factors affecting soybean growth and yield
The molecular biology research on drought resistance of WRKY transcription factor mostly focuses on Arabidopsis thaliana, Oryza sativa, Gossypium spp and other model plants and cash crops, but there is no report that GmWRKY32 transcription factor is involved in drought resistance in soybean
1.1 Bioinformatics analysis of soybean transcription factor GmWRKY32 gene 1.1.1 Analysis of conservative structural domain of soybean GmWRKY32 gene protein sequence In this study, Prosite online analysis software was used to learn that there was a WRKY conserved domain containing 62 amino acids through the protein sequence encoded by GmWRKY32, and there was a conserved amino acid sequence WRKYGQK at the N-terminus (Figure 1A)
Summary
Drought stress is one of the important environmental factors affecting soybean growth and yield. With the increase of drought events, the yield and quality of soybean have been seriously lost This fact forced us to deeply analyze the mechanism of drought and salt tolerance of soybean, and obtain the key genes of salt tolerance, providing important genetic resources for breeding new varieties of drought-tolerant soybean (Dias et al, 2016). WRKY family has been involved in the regulation of plant growth and development, biotic and abiotic stress responses by regulating downstream target genes or interacting with other proteins. The molecular biology research on drought resistance of WRKY transcription factor mostly focuses on Arabidopsis thaliana, Oryza sativa, Gossypium spp and other model plants and cash crops, but there is no report that GmWRKY32 transcription factor is involved in drought resistance in soybean. The research results can provide scientific basis and key genes for analyzing the molecular mechanism of soybean drought resistance and breeding new varieties of drought-resistant soybean by molecular breeding
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