Bioinformatics Analysis to Identify RNA-Protein Interactions in Oogenesis.

Abstract

Hundreds of RNA-binding proteins are known, but the biological functions are known for only a few of them. They regulate various aspects of RNA processing or biogenesis such as splicing, polyadenylation, and translation. Here I describe a bioinformatics approach that we developed to identify potential new mRNA target(s) of the Drosophila master sex-switch protein Sex-lethal (SXL) by combining computational analysis with genetic and biochemical investigation. This approach could be used to identify new RNA-protein interactions during oogenesis in the female germline and should be applicable to numerous other posttranscriptional regulatory events.