Bioinformatics Analysis on Functional Site and Fingerprint of CagI Protein in <i>Helicobacter pylori</i> Cag Pathogenicity Island

Abstract

[Objective] This paper aims to make a bioinformatics analysis on the functional site and fingerprint of CagI protein in helicobacter pylori cag pathogenicity island and further explore the functions of CagM protein. [Methods] Use PROSITE SCAN: http://www.expasy.ch/cgi-bin/ prosite/PSScan.cgi server to search the fingerprint sequence of CagI protein and analyze and discuss the possible functions. [Results] The search of PROSITE SCAN found that CagI protein has four kinds of functional sites: N-glycosylation site, protein kinase C phosphorylation site, casein kinase II phosphorylation site, and myristoylation acting site; the search of fingerPRINTScan and printsBLAST server found sixteen fingerprint tags of CagI protein: helicobacter pylori vacuolating toxin tag; flagellin tag; ATP synthetase δ subunit tag; helicobacter pylori outer membrane protein family tag, etc. It is predicted preliminarily that CagI protein also has these functions. [Conclusion] As a kind of secretory protein, CagI protein will be secreted to the outer membrane to form the membrane protein, which will become one part of elicobacter pylori IV type production secretion pilus involved in signal transduction and material transportation and acting as a messenger or carrier, and it many have hydrolase activity and ATP/GTP enzyme activity.*Fund program: National Nature Science Foundation Project (30870096); National Science Foundation Project in Jiangsu Colleges (08KJB310001)