Abstract
BackgroundThis study was to investigate some new pathological genes in colorectal adenocarcinoma of human.MethodsHuman colorectal adenocarcinoma tissues and normal colorectal tissues were taken and suppression subtractive hybridization (SSH) and cDNA microarray techniques were employed. From differentially expressed 86 expressed sequence tags (EST), 10 EST of the SSH were selected as seed sequence for bioinformatics analyses, semi-quantitative RT-PCR and PCR-sequencing. Each lane of semi-quantitative RT-PCR was analyzed by Q1 software.ResultsAmong these 10 EST, it has been found that ES274070, ES274071, ES274076 and ES274081 may play role in the onset of colorectal adenocarcinoma in human.ConclusionsThe ES274070, ES274071, ES274076 and ES274081 are related to the onset of human colorectal adenocarcinoma.
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