Abstract
Catalase (CAT) is an antioxidant enzyme, which plays a key role in plant development and abiotic stress response. In this study, three maize ZmCATs and three Arabidopsis AtCATs genes were identified by screening NCBI and phytozome databases. The protein characteristics, evolutionary relationship, gene structure, protein secondary and tertiary structure, gene expression at different developmental stages or under abiotic stress treatment were analyzed. The results showed that there were some similarities between CAT families from the two species. The proteins encoded by ZmCATs and AtCATs genes were hydrophobic. According to phylogenetic tree analysis, CATs gene family was divided into two subfamilies. ZmCATs and AtCATs both contain two conserved domains, and the secondary structures of ZmCATs and AtCATs protein are mainly α-helix and random coils. The cluster analysis of ZmCATs expression in different growth and development stages showed that ZmCAT1 , ZmCAT2 and ZmCAT3 were highly expressed in maize growth and development stage. Under abiotic stress, the expression of ZmCATs gene was significantly affected by temperature stress. The results of this study provide a theoretical basis for exploring the function of CAT in maize and other crops.
Highlights
Catalase (CAT) is an antioxidant enzyme, which plays a key role in plant development and abiotic stress response
The cluster analysis of ZmCATs expression in different growth and development stages showed that ZmCAT1, ZmCAT2 and ZmCAT3 were highly expressed in maize growth and development stage
The coding region of ZmCATs is between 1628~2500 bp, the number of amino acids of the protein is between 492~704 aa, the molecular weight is between 56~80 kDa, and the isoelectric point is between 6.45~9.33
Summary
Catalase (CAT) is an antioxidant enzyme, which plays a key role in plant development and abiotic stress response. Maize (Zea mays L.) is a widely grown food crop and an important energy crop and fodder that plays an important role in the national economy Abiotic stresses such as salt (Iwamoto et al, 2013), drought (Du et al, 2008), low temperature (Hackenberg et al, 2013) and high temperature (Li et al, 2013) are the main factors affecting its yield. The protein characteristics, evolutionary relationships, structural domains, secondary and tertiary structures of the ZmCATs and AtCATs gene families and the expression levels at different developmental stages were predicted and analyzed.
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