Abstract
The objective of this work was to evaluate the potential of biohydrogen production in agro-industrial residues, which were soft drink wastewater (SDW), corn steep liquor (CSL), cheese whey (CW), and expired Guaraná soft drink, using one model strain Clostridium beijerinckii ATCC 8260 and newly isolated Clostridium butyricum DEBB-B348. The agro-industrial residues were characterized, and all of them contained significant concentrations of carbohydrates such as glucose, fructose, lactose, and maltose, and in the case of CSL and CW they also contained significant concentrations of amino acids. After performing subsequent experimental designs, the significant factors were cheese whey concentration, corn steep liquor concentration, and fermentation time for C. beijerinckii, and corn steep liquor concentration and fermentation time for C. butyricum (p ≤ 0.05), with R2 of 0.950 and 0.895, respectively. The maximum hydrogen volume production was 18.5 ± 1.68 mL and 27.4 ± 1.84 mL for each strain, respectively. It is noteworthy the high yield of hydrogen produced with C. beijerinckii (235 mL H2/g COD removed) and C. butyricum (310 mL H2/g COD removed). It was also noted that CSL and/or CW-based culture media do not need to be supplemented with cysteine-HCl. These results indicate a potential hydrogen production process utilizing less expensive substrates.
Highlights
Alternative renewable energies are a transversal area that encompass many of the sustainable development goals, such as renewable energy, innovation and infrastructure, sustainable cities and communities, responsible consumption, climate action, and life on land
It is important to highlight that corn steep liquor (CSL) presented an elevated concentration of lactic acid (92.5 g L− 1) which can be used as a second carbon source for some lactic acid bacteria and species of the Clostridium genus [23, 24]
Biohydrogen is a promising alternative to the fossil fuels since it emits no greenhouse gases and its production from agro-industrial wastes significantly reduces the production costs
Summary
Alternative renewable energies are a transversal area that encompass many of the sustainable development goals, such as renewable energy, innovation and infrastructure, sustainable cities and communities, responsible consumption, climate action, and life on land. The unacceptable environmental impacts caused by the large use of fossil fuels have boosted research on sustainable biofuels with reduced environmental impacts [1]. Hydrogen (“bioH2”) stands out for not producing greenhouse gases during its combustion, for being produced from a variety of energy sources, for being safely stored and transported, and for being converted into electricity [2]. Hydrogen may play an important role in building a more sustainable society, especially in the transportation sector where CO2 capture from vehicles is virtually impossible. The renewable production of hydrogen is derived from the dissociation of H2O (commercial technology), the thermochemical processing of biomass, and the anaerobic fermentation of organic raw materials. Fermentative hydrogen production (“bioH2”) is generated under milder conditions and using (agro)industrial byproducts
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
