Abstract
The rubber latex processing wastewater (RLPWW) has not considered as an ideal source for the bioconversion. Most previous works have only focused on the technologies applied for RLPWW treatment. In this study, RLPWW was used as a substrate to produce biohydrogen gas by five anaerobic microbial mixtures isolated from sewage sludge. The results showed that the microbial mixtures can well grow in this wastewater. First step, RLPWW was pretreated by Al2(SO4)3 with dosage of 300 mg/l and pH 7 to reach suitable conditions of COD, BOD and TSS which are respectively 184, 110 and 125 mg/l. Second step, the microbial mixtures were added in pretreated RLPWW to ferment biohydrogen in continuous stirred tank reactor (CSTR). Dominant hydrogen-producing bacteria of the mixtures determined by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method were Clostridium, Citrobacter, Klebsiella and Enterobacter. After 28 hours, BPM mixture conducted a maximum cumulative hydrogen content of 221.35 ml H2/l and COD, BOD, TSS, N, and P removals of 64.38, 71.43, 65.96, 54.12, and 79.86%, respectively. The application of the studied microbial mixtures was obviously effective method to combine the biohydrogen production and the RLPWW treatment.
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