Biogenic synthesized SeONPs by Bacillus paramycoides as antimicrobial, anticancer, antioxidant, apoptotic and hepatorenal treating agent.

Abstract

Employing bacteria in nanoparticles (NPs) biosynthesis has appeared as a fast-emerging research area in green nanotechnology applications. The biosynthesis processes optimization can lead to obtaining NPs with desired sizes and suitable quantities. The goal of the current work was to synthesize selenium oxide nanoparticles (SeONPs) from Bacillus paramycoides (MCCC 1A04098), a locally isolated terrestrial bacteria, and in vitro investigate its antibacterial, anticancer, and anti-cytotoxic properties. Rats with hepatorenal injury triggered by carbon tetrachloride (CCl4) were used for the in vivo evaluation. Oxidative stress markers, lipid profile, liver and kidney function indices, as well as their histological features were measured and compared with silymarin as a reference drug. SeONPs revealed antimicrobial effect against Bacillus cereus, Staphylococcus aureus, Escherichia coli, and Candida albicans. It also showed anticancer effect on hepatic (HepG2) and breast (MCF-7) cancer cell lines in a dose dependently with apoptotic effect rather than necrosis. Treatment of CCl4 injured rats with SeONPs restored all the biochemical parameters to normal values with variable degrees and the results were confirmed by the histological pictures of liver and kidney. In conclusion, the obtained biogenic SeONPs from the local isolate of Bacillus paramycoides strain (MCCC 1A04098) showed a remarkable biomedical application including in vitro antimicrobial and anticancer activities. It also recorded in vivo antioxidant, anti-hypercholesterolemic and anti-cytoxic activities against hepatorenal injury in rats. Therefore, SeONPs synthesis by ecofriendly technique may be considered as a promising nutraceutical agent.