Biogenic Synthesis of Silver Nanoparticles using Azadirachta indica (Neem) Leaf Extract To Achieve Inhibitory Action Against Antibiotic-Resistant Bacteria

Abstract

Background: Nanotechnology is an emerging research field on nanoparticles. Nanoparticles are 1-100 nm in one dimension and are important in biomedical science, medical chemistry, atomic physics, and most other known fields. They are used because of their small size, orientation, and physical properties. Objectives: This study was designed to synthesize silver nanoparticles (AgNPs) using Azadirachta indica (Neem) leaf extract and evaluate their biomedical application. Methods: An aqueous extract of Azadirachta indica was used to synthesize AgNPs. 1 ml of the extract was added drop by drop to 30-60 ml of a 1mM solution of silver nitrate. The AgNPs were characterized by UV-Visible Spectroscopy, Fourier Transform Infrared Spectroscopy, Dynamic Light Scattering Spectroscopy, X-ray Diffraction, Transmission Electron Microscopy, and antibacterial activity studies. The biological synthesis of AgNPs was performed using the aqueous solution of Azadirachta indica leaf extract and AgNO3. A fixed ratio of plant extract to metal ion was used to prepare AgNPs and the formation of the nanoparticles was confirmed through the color change. The nanoparticles were characterized by UV-vis spectrophotometer, FTIR, DLS, XRD and TEM, and were found to range in size from 30-60 nm. Results: The biosynthesized AgNPs had a bactericidal effect against the antibiotic resistant pathogenic microorganisms Bacillus subtilis, Pseudomonas aeruginosa, and Bacillus cereus. Conclusion: Silver nanoparticles were synthesized from Azadirachta indica leaf extract and can be used as a therapeutic candidate for biomedical applications.