Biogenic Synthesis of Gold Nanoparticle from Enicostema axillare and Their In Vitro Cytotoxicity Study Against MCF-7 Cell Line

Abstract

In the present study, the green synthesis of gold nanoparticles by using Enicostema axillare (Lam) extract was employed as a reducing agent. In the green approach of gold nanoparticles is a more stable, reliable, and eco-friendly method. The structural and compound analyses of AuNPs were determined by various analytical methods like UV-Vis spectroscopy, SEM, TEM, XRD, and FTIR. The shape and size of the nanoparticles were measured between 0.5 μm and 1 nm. The reducing agent solution was maintained by UV-Vis spectroscopy analysis; the functional groups and area were analyzed ranging from 400 to 4000 cm−1 at a resolution of 4 cm−1. X-ray diffraction intense peaks at 20 values of 38°, 44°, 65°, and 77° that correspond to the indices [111], [200], [220], and [311] were recorded. AuNPs showed a decrease in cell viability with the inhibition of growth in the cancerous cell line (MCF-7). Different doses of green-synthesized AuNPs were added at concentrations of 100, 50, 25, 12.5, 6.25, 3.125, 1.562, and 0.781 μg/ml in the MCF-7 cancerous cell line. The present study reports a contribution of green synthesis of gold nanoparticles, and it can be moved to another metal.