Biogenic Synthesis of Different forms of Bio-caped Silver Nanoparticles using Microcystis sp. and its Antimicrobial Activity

Abstract

Background: Nanobiotechnology is a cutting-edge field that is revolutionizing the way we produce and utilize nanoparticles. With bacteria becoming increasingly resistant to traditional antibiotics, researchers are exploring new ways to synthesize antimicrobials. Objective:: The aim of this study was to investigate the biosynthesis of different forms of silver nanoparticles using different biological methods from Microcystis sp. to be used as an antimicrobial agent. Methods: We employed the direct strain powder method, ethanolic extract pellets, and ethanolic extract. Then a combination of analytical techniques was used to characterize the properties of nanoproperties. Finally, we evaluated the antimicrobial activity of the AgNPs against a panel of bacteria and fungi. Results: AgNPs were found in various forms, such as cubic, spherical, and rod shapes. UV-Vis detected a peak at 420 nm, and SEM identified the AgNPs with bio-capped layers ranging from 40-130 nm. The antibacterial test revealed that the pellet method produced the most effective AgNPs, specifically against Gram-positive bacteria such as Staphylococcus sp. with a clear zone of 32 mm, while the larger cubic AgNPs produced by the powder method were less effective as antibacterial agents. Conclusion: This study demonstrates that AgNPs can be produced using Microcysts sp. as a reducing and capping agent; furthermore, they are produced in different shapes as cubic, spherical, and rod shapes. Besides that, cubic nanoparticles are more effective in killing germs than spherical ones. The results of this study will help to improve our understanding of the mechanisms of AgNPs biosynthesis and pave the way for the development of new and more effective antimicrobial agents.