Abstract
Production of H2S is one of the main problems in the petroleum industry and one of the leading causes of corrosion in storage tanks. The microbial species found in marine/oil environments and tanks include the general anaerobic heterotrophic bacteria (GAHB) and the sulfate-reducing bacteria (SRB). This research implemented a method based on the semi-continuous production of sulfide, over 15 days, in an attempt to make correlations between sulfide production and microbial quantification. Observation of the most suitable conditions for biogenic-sulfide production indicated that an increase in salinity and temperature produce a significant decrease in the semi-continuous biogenic production of sulfide. Dilution of the culture medium promoted a decrease in cell growth and production of sulfides. The quantification of SRB and GAHB was evaluated using the MPN (Most Probable Number) technique according to FDA regulations (Food and Drug Administration). The use of this procedure contributed to the increase in reliability coefficient used in the quantification data.
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