Abstract
Precursors to small subunits (SSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) and apoproteins of light-harvesting chlorophyll a/b binding protein (LHCPII) in photosystem II were synthesized in vitro by expressing pine (Pinus thunbergii Parl.) cDNA clones encoding these proteins. The precursors to pine SSU and LHCPII were post-translationally transported into spinach chloroplasts, processed into mature size, and localized in only stroma and thylakoid membranes, respectively. The pine SSU was tightly integrated into spinach Rubisco; however, the pine LHCPII was not found in the light-harvesting chlorophyll–protein complex. These results suggest that a common biogenesis pathway of nuclear-encoded chloroplast proteins is present both in gymnosperms and in angiosperms.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
