Abstract
A variety of rapidly growing mammalian cells contain a substantial portion of their actin mRNA in a poly(A) − form. We have used DNA-driven hybridization of a cloned actin cDNA-containing plasmid with pulse-labeled RNA from mouse S-180 ascites cells to examine newly synthesized actin mRNA. Our results indicate that the same proportion of newly synthesized and steady-state actin mRNA (approx. 40%) exists in a poly(A)-deficient form. This suggests that the poly(A) − form arises by some process other than slow cytoplasmic de-adenylation of a poly(A) + precursor. We have also examined cell cycle-enriched populations of S-180 ascites cells for the presence of poly(A) − actin mRNA. Results from these experiments indicate that cells in G1 phase of the cell cycle contain predominantly poly(A) + actin mRNA, while the poly(A) − form is restricted to late-S and post-S phase Cells.
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