Abstract
Temperature has different effects on anaerobic digestion (AD) of various biomasses, which could bring out changes in microbial communities. The relationship between microbial community and methane production at 35 °C (R35), 38 °C (R38), 41 °C (R41), and 44 °C (R44) was analyzed during AD of corn stover (CS). The results showed that the daily biogas and methane production from R44 were 16.6%–42.4% and 16.2%–40.6% higher than yields from R35, R38 and R41, respectively. The abundance of Bacteroidetes in R35, R38 and R41 was relatively close (30.70%–39.36%), which was low in R44 (16.00%). The abundance of Firmicutes in R35 was 32.30%, however, Firmicutes was the most dominant phylum at R44 (66.58%). The abundance of Miscellaneous_Crenarchaeotic_Group and Euryarchaeota were 54.63 ± 6.47% and 44.43 ± 6.73% across all digesters. This research demonstrated that among all temperatures studied, 44 °C could enhance the conversion efficiency of the substrates to methane and be recommended for better conversion of CS in AD process.
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