Abstract
Panonychus citri is one of the most damaging pests of horticultural crops. Conventional control of this pest population through pesticides has led to the enhanced pest resistance. Management of P. citri population through RNAi, is still largely unknown. In oviparous organisms, fabrication and development of yolk protein play a vital role in the reproduction. Vitellin (Vn) is the source of eggs storage that helps in proper functioning of Vitellogenin (Vg) and Vitellogenin receptor (VgR). VgR is very compulsory protein for the development of Vg into oocytes. In the current study, Vg (PcVg) and VgR (PcVgR) genes were studied and their expressions at different developmental stages were quantified by RT-qPCR. Females treated with dsRNA of PcVg and PcVgR genes exhibited reduction in gene expression. Down regulation of target genes significantly effected oviposition and reduced the egg laying capacity up to 48% as compared to control (ds-egfp). Synergistic effect of target gene’s dsRNA was also accessed that reduced the egg laying up to 60.42%. Furthermore, combination of target dsRNA on deutonymph and protonymph also resulted in 67% and 70% reduction in eggs, respectively. Synergistic effect of dsRNA at 1000 ng/ul resulted in longer life span as compared to control treatments. This study suggests to develop a new strategy of P. citri population control by reducing its reproduction.
Highlights
Insect Vg is directly involved in the somatic pathway, and the disturbance in this pathway may lead to the decline in somatic cells of honey bees
In current study two citrus red mites, female specific genes (PcVg & PcVgR) were selected and their efficacies were observed by Double stranded RNA (dsRNA) leaf dip method
Results revealed that all the tested dsRNA of PcVg & PcVgR depicted a significant effect on female infertility
Summary
Insect (such as the honey bee) Vg is directly involved in the somatic pathway, and the disturbance in this pathway may lead to the decline in somatic cells of honey bees They declared that depletion in Vg plays the significant role in zinc-ligand, which may cause the pycnosis of haemocytes[15]. Molecular information and bio-functioning of Vg and VgR had been revealed in two spotted spider mite, Tetranychus urticae[17]. They justify the function of Vg gene by inferred amino acid sequences, which confined the von Willebrand factor D domain and the GLCG motif, which were reported to be the common features of Vg sequences in insects and ticks. It is demonstrated that RNAi is an alternative method of radioactivity and has opened the new door to deal with the challenges against other agricultural pest
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