Bioformulation of Microbial Fertilizer Based on Clay and Alginate Encapsulation.

Abstract

This study aims to develop new formulations for microbial fertilizers Pseudomonas fluorescens Ms-01 (Pf) and Azosprillum brasilense DSM1690 (Ab) using two kinds of clay minerals. The studied formulations were prepared as hybrid materials based on halloysite and alginate [Ha-Ag] or montmorillonite and alginate polymers [Mt-Ag] and were applied to the bacterial strains to develop low cost, efficient, and slow-release capsules. Their efficiency was evaluated in comparison with alginate [Ag] as the control. The produced capsules were spherical in shape and were chemically and physically characterized and further analyzed for their swelling ratios, soil biodegradability, release kinetics of microbial cells, and their survival stability over 3months of storage under different conditions (room temperature vs 4°C). The effect of the capsules on the growth of wheat plants was also investigated. Results showed that both formulations were able to preserve bacterial survival which reached 14.8log CFUg-1 after 3months storage in the halloysite formulation. The swelling ratios were ranged between 61.5 ± 1.35% and 36.5 ± 5% for the montmorillonite and the halloysite formulations, respectively. The release kinetics revealed the slow-release capacity of the capsules mainly with the halloysite formulation which significantly released bacterial cells after 15days of incubation in saline water (15.24log CFUmL-1). The application of the capsules to wheat plants significantly increased root and shoot biomasses and nitrogen content in the roots. In conclusion, halloysite minerals seem to be more adapted as additive to alginate in microbial encapsulation.