Biofilm Suppression of Pseudomonas aeruginosa by Bio-Engineered Silver Nanoparticles from Hellenia speciosa Rhizome Extract

Abstract

Bacterial biofilm, a persistent issue in healthcare equipment and typical infections, is exacerbated by the pathogenesis and antibiotic tolerance of Pseudomonas aeruginosa. This bacterium remains a significant concern in the global healthcare sector. Silver nanoparticles, with their potent antibacterial properties, have emerged as a promising solution.This study, therefore, is of utmost importance as it aims to delve into the parameters influencing the biogenic nanoparticle-assisted regulation of bacterial adherence by Pseudomonas aeruginosa. The nano-sized particles were bioengineered using Hellenia speciosa rhizome extracts, which mainly included biologically active components such as mequinol, 4-hydroxy-3-methylacetophenone, and phenol, 2,6-dimethoxy, supplemented with the formation of silver nanostructured materials. The nanoclusters were characterized by UV-Vis spectrophotometry, X-ray scattering, and scanning electron microscopy (SEM). According to a microtiter plate experiment, the nanoparticle degraded biofilms up to 94.41% at dosages varied from 0 to 25 μg/ml. The light microscopy study and the interface architecture of biofilm suppression by electron microscopy demonstrated the nano-sized particle's potential to prevent bacterial adherence.