Abstract
Introduction: Urinary Tract Infection (UTI) causes inflammation which is a common, painful and sometimes life-threatening condition as well. Despite high prevalence of bacteriuria, the information on biofilm forming bacteria is negligible.
 Objectives: This study aims at understanding the status of the biofilm forming nature of Klebsiella spp and Pseudomonas SPP and their drug resistance property with several class of antibiotics with a prime focus on resistance pattern against few penicillin based drugs to few empirical drugs in today's time.
 Methodology: Urine samples were analyzed and the isolates were biochemically identified. Then, the isolates were tested for several drugs so as to identify multidrug resistance nature of isolates by Kirby-Bauer Disc method. Biofilm forming nature was examined on Congo Red Agar.
 Results Out of 35 urine samples, 13 isolates (37.1%) were found to be positive with significant bacteriuria. Eight samples (22.8%) showed incidence of Klebsiella spp. and 5 samples (14.3%) showed P. aeruginosa. The prevalence of Klebsiella spp. (Klebsiella pneumoniae (46.2%) and K. oxytoca(15.4%)) and P. aeruginosa was found to be 61.4% and 38.6% respectively. 66.6% of K. pneumoniae and 50% K. oxytocawere biofilm forming pathogen.K. pneumoniae andK. oxytoca were resistant to amoxycillin, amoxycillin-clavulanate, and cefoxitin; while were sensitive to nitrofurantoin and azithromycin.P. aeruginosa were sensitive to azithromycin(100%), but showed 60% resistance to levofloxacin and ofloxacin. Eight (61.5%) isolates were found to be MDR. 100% of Klebsiella oxytoca (n=2), 66.7% of Klebsiella pneumoniae (n=4), and 40% of Pseudomonas aeruginosa (n=2)were multidrug resistant (MDR).Multiple antibiotic resistance (MAR) indices of bacteria revealed that all the 13 isolates were Multi-Antibiotics Resistance strains.
 Conclusion Biofilm forming nature is now much greater in Klebsiella spp; while most of the isolates like Klebsiella and Pseudomon as are multidrug resistant.
Highlights
Urinary Tract Infec on (UTI) with a pathogen causes inflamma on which is a common, distressing and occasionally life-threatening condi on
Biofilm forming nature was examined on Congo Red Agar
K. pneumoniae and K. oxytoca were resistant to amoxycillin, amoxycillin-clavulanate, and cefoxi n; while were sensi ve to nitrofurantoin and azithromycin
Summary
Urinary Tract Infec on (UTI) with a pathogen causes inflamma on which is a common, distressing and occasionally life-threatening condi on. UTI affects people of all ages and both genders. Female are more suscep ble to UTIs compared to male.[1] Worldwide about 150 million people are diagnosed with UTIs each year.[2,3] In sexually ac ve healthy female pa ents with structurally and func onally normal urinary tracts may have uncomplicated UTIs.[4] Complicated UTIs are associated with co-morbid condi ons that prolong the need for treatment like abnormali es of the urinary tract, foreign body presence like indwelling catheter or stone, and infec on with MDR pathogens.[5] Signs and symptoms may include fever, chill, dysuria, urinary urgency, frequent and cloudy or malodorous urine
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