Biofilm Production and Molecular Epidemiology of P. aeruginosa by RAPD Isolated from Intravascular Catheters in Tertiary Care Hospital

Abstract

P. aeuroginosa, various culture media such as Brain Heart Infusion Agar, Citrimide Agar and Triple Sugar Iron Agar were used. Antibiotic sensitivity of the isolated strains was determined by disk diffusion test using Muller-hinton Agar medium. Moreover, antiseptic sensitivity of the strains to Decosept and Povidone-Iodine as the main antiseptic agents was identified by determining Minimal Bactericidal Concentration and Minimal Inhibitory Concentration using Nutrient Agar and Nutrient Broth media. Results: Fifty-five of the total 292 samples were positive for P. aeuroginosa. Based on the ratio of the number of positive samples to the total samples, Thalassemia ward (38.5%) was the most infected ward and taps (61.1%) was the most infected sampling place. None of the samples from room atmosphere and intensive care equipments were positive for P. aeuroginosa. Twenty percent of the samples were resistant to ceftazidim, 32.7% resistant to piperacillin, and 10.9% to both ceftazidim and piperacillin. Conclusion: Results of the study showed that contamination with P. aeuroginosa and antibiotic resistance of the isolated bacteria are the main challenges that this medical center is facing. Taking appropriate preventive measures such as using suitable antiseptic materials and installing pedals for tap waters could have major impacts on reducing hospital contamination with P.aeuroginosa and possibly other bacteria.