Biofilm production and antibiotic resistance pattern among bacterial isolates from wound samples in a rural tertiary care teaching hospital

Abstract

Pyogenic wound infections are the one of the leading cause of morbidity and mortality worldwide. Some of the common etiological agents responsible are ., and . The antimicrobial resistance has become a global challenge and the resistant pathogen poses a grave threat to the public health worldwide. Pyogenic bacteria producing biofilm has a potential to cause significant mortality and morbidity in human. The present study was carried out to determine the bacteriological spectrum of wound infections and their antibiogram to commonly used antibiotics and to detect the biofilm production by the isolates. This cross sectional study was carried out in the department of Microbiology, Adichunchanagiri institute of Medical sciences from September 2016 to August 2017. Two hundred and forty samples from various wounds were collected and processed as per standard procedures and biofilm production was detected by Congo red agar method.Out of 240 pus isolates, species were the most commonly isolated (48.85%) followed by species (11.7%). Biofilm was produced by 49.2% isolates .Majority of Gram negative bacilli were susceptible to Colistin (100%) followed by Tigecycline (Biofilm producers 75%, biofilm non producers 66.7%).All Gram positive isolates were susceptible to Vancomycin and Teicoplanin (100%) followed by Linezolid (biofilm producer 98.8%, biofilm non producer 97.8%) . Routine surveillance for wound infections along with early identification and adopting efficient control protocol against biofilm forming organism plays an important role in the prevention of the most serious infections.