Abstract
Objectives: To evaluate the antibiofilm potential of Terminalia arjuna and Ipomea carnea plant extract against potent biofilm forming UTI pathogens. Methods/Statistical analysis: In this study, previously isolated and characterized three UTI pathogens viz. Escherichia coli, Pseudomonas aeruginosa and Staphylococcus equorum were used to evaluate antibiofilm potential of selected plants. Soxhlet apparatus was used with a solvent methanol for the extraction process. For the evaluation of antibiofilm activity of different concentrations (2, 4, 6, 8 and 10 mg/ml) of plant extract in 3% DMSO Crystal violet assay was used. Findings: The methanolic bark extract of T. arjuna showed maximum activity up to 89.84% at 10mg/ml. against S. equorum while leaves extract of I. carnea gives maximum antibiofilm activity up to 67.53% at 2mg/ml against E. coli. The result shows that the investigated plants might be helpful in the development of potent herbal drugs to treat UTI. Keywords: T. arjuna; I. carnea; E. coli; P. aeruginosa; S. equorum; Soxhletextraction; Crystal violet assay
Highlights
Urinary tract infections (UTIs) are the most common human infectious disease affecting the bladder, kidneys and urinary tracts[1]
Plants are a potential source of antimicrobial compounds and several researchers throughout the world are investigating the antimicrobial activity of medicinal plants, which are utilized in the traditional or alternative healthcare systems[21]
Two important plant varieties namely T. arjuna and I. carnea were selected in the present study because they are very found in large no. in the region of Baramati, Pune (Maharashtra)
Summary
Urinary tract infections (UTIs) are the most common human infectious disease affecting the bladder, kidneys and urinary tracts[1]. Escherichia coli is the most frequent pathogen causing UTI in humans and one of the most common causes of Gram-negative bacteremia in hospitalized patients[2]. Other bacteria involved Proteus mirabilis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus spp., Enterobacter spp., group B Streptococcus and S. saprophyticus[3]. The cells embedded in a self-produced extracellular polymeric matrix produces microbial biofilms. They are the result of complex intra and inter cellular signaling and communication processes, regulated by a complex quorum sensing (QS) regulation system, which are ubiquitous in the microbial world[5]. Since biofilms interfere with the action of several antibiotics and bacterial drugs, biofilm-forming bacteria are more difficult to eradicate[6]
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