Abstract
Methicillin-resistant Staphylococcus aureus is a major human pathogen that poses a high risk to patients due to the development of biofilm. Biofilms, are complex biological systems difficult to treat by conventional antibiotic therapy, which contributes to >80% of humans infections. In this report, we examined the antibacterial activity of Origanum majorana, Rosmarinus officinalis, and Thymus zygis medicinal plant essential oils against MRSA clinical isolates using disc diffusion and MIC methods. Moreover, biofilm inhibition and eradication activities of oils were evaluated by crystal violet. Gas chromatography–mass spectrometry analysis revealed variations between oils in terms of component numbers in addition to their percentages. Antibacterial activity testing showed a strong effect of these oils against MRSA isolates, and T. zygis had the highest activity succeeded by O. majorana and R. officinalis. Investigated oils demonstrated high biofilm inhibition and eradication actions, with the percentage of inhibition ranging from 10.20 to 95.91%, and the percentage of eradication ranging from 12.65 to 98.01%. O. majorana oil had the highest biofilm inhibition and eradication activities. Accordingly, oils revealed powerful antibacterial and antibiofilm activities against MRSA isolates and could be a good alternative for antibiotics substitution.
Highlights
Methicillin-resistant Staphylococcus aureus (MRSA) is considered a principal human pathogen and the most common cause of nosocomial infections
This study aimed to investigate the antibacterial, biofilm inhibition, and eradication properties of O. majorana, R. officinalis, and T. zygis medicinal plants’ essentials oils (EOs) against clinical methicillin-resistant
The outcomes of this study indicated that O. majorana EO had the greatest antibiofilm activity against MRSA isolates succeeded by R. officinalis and T. zygis
Summary
Methicillin-resistant Staphylococcus aureus (MRSA) is considered a principal human pathogen and the most common cause of nosocomial infections. MRSA causes several diseases ranging from skin and soft tissue infections to serious invasive infections such as pneumonia, bacteremia, endocarditis and osteomyelitis [1]. The number of MRSA infections, which are more frequently associated with mortality than other bacterial infections, has increased considerably over recent years. Biofilm is considered as major virulence factor and is an organized structure built by almost all bacteria that is composed of nucleic acids, lipids, proteins, and polysaccharides [4]. Biofilms contribute to >80% of human infections and S. aureus is considered as the leading species in biofilm-associated infections [5]. In Biofilm, MRSA like other bacteria, become more persistent in the host organism, environment, and medical
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
