Abstract
ABSTRACT: Prototheca spp. have been reported as an emergent environmental mastitis pathogen in several countries. Biofilm formation is a significant factor associated with different degrees of virulence developed by many microorganisms, including Prototheca spp. The present study aimed to compare two growth conditions and two staining dyes to determine which combination was more appropriate to evaluate qualitatively and quantitatively the production of biofilm by P. zopfii. Biofilm formation was evaluated in polystyrene microplates under static and dynamic growth conditions and staining with crystal violet or cotton blue dye. All P. zopfii isolates from cows with mastitis were classified as biofilm-producers in all growth conditions and staining. The cotton blue dye proved to be more appropriate method to classify the intensity of P. zopfii biofilm production.
Highlights
Formação de biofilme por Prototheca zopfii isolados de mastite bovina clínica e subclínica em distintas condições de crescimento sob diferentes corantes
The algae’s poor response to conventional therapy has long been known (COSTA et al, 2004), only recently it has been demonstrated that the biofilm formed by P. zopfii is associated with microorganism persistence in the mammary gland, and to antimicrobial resistance (MORANDI et al, 2016)
Thirty-two strains of P. zopfii isolated from cows with clinical (n=27) and subclinical (n=5) mastitis were used in this study
Summary
Formação de biofilme por Prototheca zopfii isolados de mastite bovina clínica e subclínica em distintas condições de crescimento sob diferentes corantes. Prototheca spp. are unicellular achlorophyllous algae that reproduce asexually (SUDMAN & KAPLAN, 1973), and are widely distributed in the environment, especially in the presence of water and organic matter (PORE et al, 1983). These microorganisms have been isolated from diverse animal species, from dairy cattle. The algae’s poor response to conventional therapy has long been known (COSTA et al, 2004), only recently it has been demonstrated that the biofilm formed by P. zopfii is associated with microorganism persistence in the mammary gland, and to antimicrobial resistance (MORANDI et al, 2016). Given the relevance of this issue, the aim was to compare two growth conditions and two staining dyes to determine which combination was more appropriate to evaluate qualitatively and quantitatively the production of biofilm by P. zopfii
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