Biofabrication of Electrospun Scaffolds for the Regeneration of Tendons and Ligaments.

Alberto Sensini,Luca Cristofolini

doi:10.3390/ma11101963

Abstract

Tendon and ligament tissue regeneration and replacement are complex since scaffolds need to guarantee an adequate hierarchical structured morphology, and non-linear mechanical properties. Moreover, to guide the cells’ proliferation and tissue re-growth, scaffolds must provide a fibrous texture mimicking the typical of the arrangement of the collagen in the extracellular matrix of these tissues. Among the different techniques to produce scaffolds, electrospinning is one of the most promising, thanks to its ability to produce fibers of nanometric size. This manuscript aims to provide an overview to researchers approaching the field of repair and regeneration of tendons and ligaments. To clarify the general requirements of electrospun scaffolds, the first part of this manuscript presents a general overview concerning tendons’ and ligaments’ structure and mechanical properties. The different types of polymers, blends and particles most frequently used for tendon and ligament tissue engineering are summarized. Furthermore, the focus of the review is on describing the different possible electrospinning setups and processes to obtain different nanofibrous structures, such as mats, bundles, yarns and more complex hierarchical assemblies. Finally, an overview concerning how these technologies are exploited to produce electrospun scaffolds for tendon and ligament tissue applications is reported together with the main findings and outcomes.

Highlights

In the last three decades the topic of tissue regeneration is getting extreme attention in the orthopedic research field [1,2,3]
Among the various techniques proposed in the literature to produce scaffolds, electrospinning, and its ability to produce nanofibers, is definitely one of the most promising for tendon and ligament tissue engineering
Papers relevant to the electrospinning methodologies to produce scaffolds and their applications related to tendons and ligaments regeneration and replacement, published between 1990 and 31 July 2018, were selected:

Summary

Introduction

In the last three decades the topic of tissue regeneration is getting extreme attention in the orthopedic research field [1,2,3]. The use of scaffolds allows driving cells to proliferate and regenerate tissues in specific directions [4]. This property is fundamental to produce devices able to guide cells to the regeneration of the collagen anisotropy in the musculoskeletal tissues, such as tendons or ligaments [5,6,7]. Due to the low vascularization, the hypocellularity, the anisotropy and the non-linear mechanical properties of these tissues, natural tendon and ligament regeneration is complex [8]. Several works are published annually on this topic, presenting electrospun scaffolds with increasingly improved biomimicry and enhanced cellular response [9,10,11]

Objectives

Methods

Findings

Conclusion