Abstract
Superparamagnetic iron oxide nanoparticles (SPIONs) have been investigated for wide variety of applications. Their unique properties render them highly applicable as MRI contrast agents, in magnetic hyperthermia or targeted drug delivery. SPIONs surface properties affect a whole array of parameters such as: solubility, toxicity, stability, biodistribution etc. Therefore, progress in the field of SPIONs surface functionalization is crucial for further development of therapeutic or diagnostic agents. In this study, SPIONs were synthesized by thermal decomposition of iron (III) acetylacetonate Fe(acac)3 and functionalized with dihexadecyl phosphate (DHP) via phase transfer. Bioactivity of the SPION-DHP was assessed on SW1353 and TCam-2 cancer derived cell lines. The following test were conducted: cytotoxicity and proliferation assay, reactive oxygen species (ROS) assay, SPIONs uptake (via Iron Staining and ICP-MS), expression analysis of the following genes: alkaline phosphatase (ALPL); ferritin light chain (FTL); serine/threonine protein phosphatase 2A (PP2A); protein tyrosine phosphatase non-receptor type 11 (PTPN11); transferrin receptor 1 (TFRC) via RT-qPCR. SPION-DHP nanoparticles were successfully obtained and did not reveal significant cytotoxicity in the range of tested concentrations. ROS generation was elevated, however not correlated with the concentrations. Gene expression profile was slightly altered only in SW1353 cells.
Highlights
This study presents a ready-to-use protocol for iron oxide nanoparticles functionalization with dihexadecyl phosphate
Gene expression profile was slightly altered only in SW1353 cells. This preliminary investigation indicates that superparamagnetic iron oxide nanoparticles (SPIONs)-dihexadecyl phosphate (DHP) hold a great potential for biological applications
Oleic acid, Iron (III) acetylacetonate (97%), Dihexadecyl phosphate, 1-octadecene (90%), 2-butanol (95,5%), Sodium dodecyl sulfate (99%), Paraformaldehyde (95%) were purchased from Sigma-Aldrich (Sigma-Aldrich, MO, USA)
Summary
The aim of this study is to provide a detailed protocol for SPIONs functionalization with dihexadecyl phosphate (DHP) and broad biological assessment of its interactions with human cells
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