Bio-ethanol production through simultaneous saccharification and co-fermentation (SSCF) of a low-moisture anhydrous ammonia (LMAA)-pretreated napiegrass (Pennisetum purpureum Schumach).

Masahide Yasuda,Hayato Nagai,Kazuyoshi Ohta,Keisuke Takeo,Yasuyuki Ishii

doi:10.1186/2193-1801-3-333

Masahide Yasuda, Hayato Nagai + Show 3 more

Open Access

https://doi.org/10.1186/2193-1801-3-333

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Journal: SpringerPlus	Publication Date: Jul 1, 2014
Citations: 46	License type: CC BY 4.0

Affiliation: University of Miyazaki

Abstract

Efficient bio-ethanol production from napiegrass (Pennisetum purpureum Schumach) was investigated. A low-moisture anhydrous ammonia (LMAA)-pretreated napiegrass was subjected to simultaneous saccharification and co-fermentation (SSCF), which was performed at 36°C using Escherichia coli KO11, Saccharomyces cerevisiae, cellulase, and xylanase. It was found that use of xylanase as well as the LMAA-pretreatment was effective for the SSCF. After the SSCF for 96 h, the ethanol yield reached 74% of the theoretical yield based on the glucan (397 mg g-1) and xylan (214 mg g-1) occurring in dry powdered LMAA-pretreated napiergrass.

Highlights

Bioethanol from lignocellulosic biomass has been recognized as one of promising sustainable energy source alternative to petroleum-based fuels, since the lignocelluloses are not directly in competition with food sources (Galbe and Zacchi 2007; Taherzadeh and Karimi 2007)
We applied Simultaneous saccharification and co-fermentation (SSCF) to ethanol production from napiergrass, which was treated by low-moisture anhydrous ammonia (LMAA) pretreatment
We have previously examined ethanol formation through a combination of simultaneous saccharification and fermentation (SSF) using enzyme and S. cerevisiae with a pentose fermentation using E. coli KO11

Summary

Introduction

Bioethanol from lignocellulosic biomass has been recognized as one of promising sustainable energy source alternative to petroleum-based fuels, since the lignocelluloses are not directly in competition with food sources (Galbe and Zacchi 2007; Taherzadeh and Karimi 2007). We are interested in ethanol production from herbaceous lignocellulosic napiergrass (Pennisetum purpureum Schumach) because of its low lignin content and high harvest amount per year and per area (Yasuda et al 2012, 2013). Pentose fermentation is an unavoidable process because of its high xylan content. Ethanol concentration is usually too low (

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