Bioethanol production from microwave-assisted acid or alkali-pretreated agricultural residues of cassava using separate hydrolysis and fermentation (SHF).

Abstract

The effect of microwave (MW)-assisted acid or alkali pretreatment (300W, 7min) followed by saccharification with a triple enzyme cocktail (Cellic, Optimash BG and Stargen) with or without detoxification mix on ethanol production from three cassava residues (stems, leaves and peels) by Saccharomyces cerevisiae was investigated. Significantly higher fermentable sugar yields (54.58, 47.39 and 64.06g/L from stems, leaves and peels, respectively) were obtained after 120h saccharification from MW-assisted alkali-pretreated systems supplemented (D+) with detoxification chemicals (Tween 20+polyethylene glycol 4000+sodium borohydride) compared to the non-supplemented (D0) or MW-assisted acid-pretreated systems. The percentage utilization of reducing sugars during fermentation (48h) was also the highest (91.02, 87.16 and 89.71%, respectively, for stems, leaves and peels) for the MW-assisted alkali-pretreated (D+) systems. HPLC sugar profile indicated that glucose was the predominant monosaccharide in the hydrolysates from this system. Highest ethanol yields (YE, g/g), fermentation efficiency (%) and volumetric ethanol productivity (g/L/h) of 0.401, 78.49 and 0.449 (stems), 0.397, 77.71 and 0.341 (leaves) and 0.433, 84.65 and 0.518 (peels) were also obtained for this system. The highest ethanol yields (ml/kg dry biomass) of ca. 263, 200 and 303, respectively, for stems, leaves and peels from the MW-assisted alkali pretreatment (D+) indicated that this was the most effective pretreatment for cassava residues.