Abstract
The possibility of producing bioethanol from the biomass of finger millet straw was studied. The effects of temperature, acid concentration, hydrolysis time, and substrate concentration were investigated. The result showed that a maximum sugar content of 79.04 and 82.01 %w/w was achieved using phenol-sulfuric acid and Fehling method, respectively, from hydrolysis of 10 % biomass concentration at 2 % sulfuric acid, 35oC reaction temperature, and 4 days of hydrolysis time. The optimized hydrolyzate sample was fermented at optimized pH 6.0, 4 g/L yeast concentration, 32.5 oC reaction temperature, 4 days of fermentation time, and maximum of 7.28 %w/v of ethanol content was obtained using Pycnometer measurement. In general, the bioethanol achieved from FMS (7.28 %) at optimized conditions were highly promising and hence, it can be employed as an alternative lignocellulosic feedstock for bioethanol production rather than using food crops such as corn, sugarcane, etc.Keywords: Acid hydrolysis, bioethanol, finger millet straw, fermentation, Saccharomyces cerevisiae
Highlights
Ethanol production through biotechnological methods has acquired considerable interest due to possible utilization of bioethanol as an alternative fuel
The maximum sugar content of 68.72 and 70.65% by phenol-sulfuric acid and Fehling method, respectively, was produced using 2% acid hydrolysate of FMS with minimum yield at 0% acid concentration (27.64%). This shows that 2 % sulfuric acid hydrolysis is more effective in simple sugar production as compared to 1, 3, and 4 % sulfuric acid hydrolysis
In this study the maximum reduced sugar from finger millet straw was achieved at 2 % sulfuric acid, which is an optimum condition in acid concentration
Summary
Ethanol production through biotechnological methods has acquired considerable interest due to possible utilization of bioethanol as an alternative fuel. The highest sugar content (about 67.04 and 67.52 %w/w by phenol-sulfuric acid and Fehling method, respectively) was obtained at 10% biomass concentration.
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