Abstract
The present study deals with isolation of novel indigenous yeast strains and their use in conversion of lignocellulosic agricultural and household waste into bioethanol. 11 yeast cultures were isolated from different sources and from these four strains of yeast were selected on basis of bioethanol production. Biochemical tests were performed to characterize the isolated yeast cultures. The identification of the unknown yeast strains was then done using the 26S rRNA gene sequence analysis. The yeast cultures were identified as: Pichia farinose, Arxula adeninivorans, Rhodotorula colostri, Stephanoascus ciferrii. These strains were inoculated in fermentation media having lignocellulosic household and agricultural waste in a 3 L fermenter. After 48 h all the four yeast strains converted the lignocellulosic waste to different amounts of bioethanol. Pichia farinose was found to produce a maximum amount of bioethanol, i.e., 31 g/L and Stephanoascus ciferrii produced 28.73 g/L bioethanol when bagasse was used as the carbon source.
Highlights
Global concerns about climatic changes and the resultant need to reduce greenhouse gas emissions have encouraged the use of bioethanol as a gasoline replacement or as an additive [1]
Many investigators have studied the production of bioethanol using only Saccharomyces cerevisiae with different raw materials such as agricultural wastes, municipal wastes [3], the fruit wastes like papaya [4] banana peels [5], pineapple [6] and grapes [7]
Total reducing sugar was measured by the dinitrosalicylic acid (DNS) method [9]
Summary
Global concerns about climatic changes and the resultant need to reduce greenhouse gas emissions have encouraged the use of bioethanol as a gasoline replacement or as an additive [1]. The Indian Government has enforced and deferred the 5% mandatory bioethanol blending law a couple of times since 2003. Apart from sugarcane, molasses and grains; no other viable biomass to bioethanol technology exists in India. Many investigators have studied the production of bioethanol using only Saccharomyces cerevisiae with different raw materials such as agricultural wastes, municipal wastes [3], the fruit wastes like papaya [4] banana peels [5], pineapple [6] and grapes [7]. It is been well established that bioethanol can be produced by different classes of yeasts. Different yeast strains would result in different levels of productivity of bioethanol. Bioethanol production depends on microbial activity, that of yeasts
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