Abstract
Effects of mixed cultures of Saccharomyces cerevisiae Levuline FB — higher ethanol tolerance — and different Kluyveromyces strains — higher inulinase activity — on the production of ethanol from Jerusalem artichoke extract were investigated. Among the investigated strains, combination of S. cerevisiae and K. marxianus strain Y00959 with simultaneous saccharification and fermentation gave the best efficiency (76%) of bioconversion. The optimal ratio of mixed cultures was determined to be 1:1 of K. marxianus and S. cerevisiae. Central composite design (CCD) was adapted to find the optimum initial substrate concentration and inoculum size for the maximal production of ethanol from Jerusalem artichoke juice. The optimum fermentation conditions were found to be 24% (m/w) substrate concentration and 45 OD600nm ml/100 ml inoculum size of mixed culture. Use of these conditions, about 10.67% (v/v) was produced at 148 h of alcoholic fermentation given. Results of this work provide benefits of mixed culture on produ...
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