Abstract
CRISPR activation (CRISPRa) in bacteria is an attractive method for programmable gene activation. In article number 1900252, Yang Liu and Baojun Wang summarize the current state-of-the-art in this area of high potential, and present the powerful features and capabilities of a newly reported eukaryote-like, σ54-dependent CRISPRa system. The eukaryote-like bacterial CRISPRa enables novel designs in synthetic gene regulation and promotes research in the σ54-dependent gene networks with broad applications envisioned.
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