Bioengineered silver nanobowls using Trichoderma viride and its antibacterial activity against gram-positive and gram-negative bacteria

Abstract

Myconanotechnology is a study of nanoparticle synthesis using fungi and their application mainly in medicine. Myconanotechnology has great advantage due to wide range and diversity of the fungi. Recently, fungal-mediated synthesis of nanoparticles is a reliable and ecofriendly method. The present work investigates the synthesis of silver nanoparticles using Trichoderma viride which is a non-pathogenic fungus. The cell filtrate of T. viride was used for the reduction of silver nitrate to silver nanoparticles. The pH of the cell filtrate was changed, and the effect of the pH was monitored on the synthesis of the silver nanoparticles. While changing the pH of the medium, the shape and size of the nanoparticles were controlled. The silver nanobowls were synthesized at first time using T. viride. The synthesized silver nanoparticles were characterized using UV spectrophotometer, X-ray diffractometer, Fourier transform infrared spectrophotometer, scanning Electron microscope (SEM), and transmission electron microscope (TEM). The SEM and TEM images showed the shape and size of the nanoparticles, and the synthesized nanoparticles were nanobowl in shape and polydispersed in size. The antibacterial activity of silver nanoparticles was carried out against gram-positive and gram-negative organisms. The maximum zone of inhibition occurred at 50 μl, while the concentration of the nanoparticles increased the inhibition activity also increased.