Bioenergy grass [Erianthus ravennae (L.) Beauv.] secretes two members of mugineic acid family phytosiderophores which involved in their tolerance to Fe deficiency

Abstract

ABSTRACTRavenna grass, Erianthus ravennae (L.) Beauv. (E. ravennae) is a potential high biomass-energy crop with low input requirements. Iron (Fe) deficiency in calcareous soils is a widespread agronomic problem which reduces crop yields. Fe is sparingly soluble under aerobic conditions at high soil pH, such as in calcareous soils; therefore, plants cannot take up enough Fe. Increasing crop productivity of giant grasses, such as Ravenna grass in calcareous soil, has a positive effect by alleviating environmental problems. However, the growth character in calcareous soil and Fe homeostatic trait of Ravenna grass are largely unknown. In this study, we analyzed characteristics of Ravenna grass. The growth of E. ravennae plants were impaired in calcareous soil compared to those in the normal soil. In calcareous soil, the growth of E. ravennae plants differ among the water and fertilizer conditions; E. ravennae plants were grown better in the submerged condition adding micronutrient among conditions. These results suggested that impaired growth of E. ravennae in calcareous soil might be micronutrient shortage. We found that E. ravennae roots possess Fe reductase activities which were upregulated under Fe-deficient conditions. E. ravennae produced and secreted mugineic acid (MA) and deoxymugineic acid (DMA) to acquire Fe from the soil. The amount of MA was higher than that of DMA. Thus, E. ravennae might have both partial Strategy-I and Strategy-II Fe uptake systems. E. ravennae intercropped with transgenic rice plants producing and secreting MA through the introduction of the barley MA synthase gene showed improved growth compared to monocropped E. ravennae plants, suggesting that the increased amounts of MA enhanced their tolerance to Fe deficiency. Our results suggest that there is a considerable potential to improve the growth of E. ravennae plants in calcareous soils by enhancement of their Fe uptake systems through increase of MA production.