Abstract
BackgroundCardio myoblast generation from conventional approaches is laborious and time-consuming. We present a bioelectronics on-a-chip for stimulating cells cardio myoblast proliferation during culture.MethodThe bioelectronics chip fabrication methodology involves two different process. In the first step, an aluminum layer of 200 nm is deposited over a soda-lime glass substrate using physical vapor deposition and selectively removed using a Q-switched Nd:YVO4 laser to create the electric tracks. To perform the experiments, we developed a biochip composed of a cell culture chamber fabricated with polydimethylsiloxane (PDMS) with a glass coverslip or a cell culture dish placed over the electric circuit tracks. By using such a glass cover slip or cell culture dish we avoid any toxic reactions caused by electrodes in the culture or may be degraded by electrochemical reactions with the cell medium, which is crucial to determine the effective cell-device coupling.ResultsThe chip was used to study the effect of electric field stimulation of Rat ventricular cardiomyoblasts cells (H9c2). Results shows a remarkable increase in the number of H9c2 cells for the stimulated samples, where after 72 h the cell density double the cell density of control samples.ConclusionsCell proliferation of Rat ventricular cardiomyoblasts cells (H9c2) using the bioelectronics-on-a-chip was enhanced upon the electrical stimulation. The dependence on the geometrical characteristics of the electric circuit on the peak value and homogeneity of the electric field generated are analyzed and proper parameters to ensure a homogeneous electric field at the cell culture chamber are obtained. It can also be observed a high dependence of the electric field on the geometry of the electrostimulator circuit tracks and envisage the potential applications on electrophysiology studies, monitoring and modulate cellular behavior through the application of electric fields.
Highlights
Cardio myoblast generation from conventional approaches is laborious and time-consuming
The chip was used to study the effect of electric field stimulation of Rat ventricular cardiomyoblasts cells (H9c2)
Cell proliferation of Rat ventricular cardiomyoblasts cells (H9c2) using the bioelectronics-on-a-chip was enhanced upon the electrical stimulation
Summary
Cardio myoblast generation from conventional approaches is laborious and time-consuming. Applications can range from studying the growth and information processing of neurons [10] or the effect of electric impulses in cardiac cells [11], capillary electrophoresis chips for the separation of biochemicals such as amino acids and nucleotides [12] up microstructures for the analysis of DNA [13] Such electrical stimulators present the same assembly that consist of two electrodes which are directly in contact with cells and culture medium and the electric impulse is externally applied [14,15,16,17]. Once you have a thin film of material only the laser interaction is needed to fabricate the electrode, instead of the chemical components This technique uses the process of laser ablation, where the interaction of the laser energy with the sample leads to material removal. The use of ultrafast lasers avoids this approach since ablation takes place as result of multi-photon absorption at high peak intensities, which means that even materials normally transparent to the laser wavelength can be processed [29]
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