Abstract
A new, simple amperometric sensor system for detecting phenols has been developed. Using the electrochemical doping method, a polyaniline tyrosinase electrode (TS/PAN/Pt) has been devised as a detecting phenol sensor. The effect of experimental parameters, such as operating potential, pH, and temperature, has been studied. The activation energy of the enzyme-catalyzed reaction is 14.73 kJ mol−1. Comparison of the responses with the 4-aminoantipyrine standard method for phenol sample analysis indicated the feasibility of the TS/PAN/Pt sensor. The TS/PAN/Pt shows a high operational stability. Under the optimum conditions, the TS/PAN/Pt displayed detection limits for phenol in the lower nanomolar range.
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