Abstract
For the first time the data on the distribution of the potential (ϕm) and resistance (Rm) in the plasma membrane and cell wall (R0) of Chara fragilis cells were obtained using the Hogg method by the number of C. fragilis cells in standard conditions. The Hogg method allows simultaneous measurement of electrophysiological parameters, such as ϕm and Rm. The stationary values of the membrane potential and resistance varied in a rather wide range: –90...–300 mV and 1.0–32.6 Ohm·m2, respectively. The average values of ϕm were –183.0 ± 4.9 mV, Rm –9.0 ± 1.2 Ohm·m2. Using standard modifiers of membrane transport, the electrogenic activity of the cells was differentiated into two types: K+-channels and H+-membrane pumps. The activation ranges of the K+-inward rectifying channels and K+-outward rectifying channels are –130...–50 and –300...–162 mV, respectively. The cytosolic activity of K+-ions was 61.6 mmol/L. Since in our research, C. fragilis cells were studied for the first time, their electrogenic activity and ionic conductivity were tested using ammonium metavanadate (VO3ˉ) – a proton pump inhibitor and tetraethylammonium (TEA+) – a universal blocker of K+-channels of plasma membranes. Chara fragilis cells can be recommended as a test object for establishing the mechanisms of changes in plasma membrane transport under exogenous stress factors influence.
Highlights
Experimental studies of Chara algae contributed to establishing the components and kinetic characteristics of primary active transport in the plasma membrane of plant cells (Yurin et al, 1991; VĂ©ry, Sentenac, 2003; Volkov, 2006), and to revealing the mechanisms of modification of membrane transport by a number of xenobiotics, pharmacological agents, etc. (Musayev et al, 2009; Musayev, 2012)
Since the C. fragilis cells were first introduced by us in the practice of electrophysiological research, we needed to conduct a statistical analysis of the distribution of electrophysiological parameters by the number of cells in standard conditions
Based on the results reported in this study, it can be proposed that the interstitial cells of Chara fragilis with stable electrophysiological parameters can serve as a new promising object for the investigation of the membranotropic actions of exogenous stimuli, including xenobiotics, nanoparticles, phytoprotectors, pharmacological agents, etc
Summary
Experimental studies of Chara algae contributed to establishing the components and kinetic characteristics of primary active transport in the plasma membrane of plant cells (Yurin et al, 1991; VĂ©ry, Sentenac, 2003; Volkov, 2006), and to revealing the mechanisms of modification of membrane transport by a number of xenobiotics, pharmacological agents, etc. (Musayev et al, 2009; Musayev, 2012). Experimental studies of Chara algae contributed to establishing the components and kinetic characteristics of primary active transport in the plasma membrane of plant cells (Yurin et al, 1991; VĂ©ry, Sentenac, 2003; Volkov, 2006), and to revealing the mechanisms of modification of membrane transport by a number of xenobiotics, pharmacological agents, etc. Isolated Chara cells are a very promising tool for detecting adaptive reactions during the modification of membrane transport in the plasma membrane under stress (Demidchik, 2012). To this end, the involvement of new objects with different electrochemical and kinetic characteristics could stimulate identifying meaningful patterns of the response of isolated cells and plants in general to exogenous stimuli. Using specific modifiers of the transport properties of the plasma membrane, the components of the primary active transport and electrogenic activity of C. fragilis cells were identified
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