BIOEFFICACY OF SESBANIA GRANDIFLORA LEAVES SILVER NANOPARTICLES AGAINST AEDES AEGYPTI LARVAE

Abstract

The present study evaluated the green synthesis of silver nanoparticles using the leaf extract Sesbania grandiflora and charecterized by the UV-Vis Spectrophotometry, Particle size analyzer and Fourier Transform Infra-Red Spectroscopy (FTIR) and also evaluated its killing effect against larval and pupal stages of Aedes aegypti. The production of silver nanoparticles was observed by the colour changes from yellow to dark brown and further confirmed by UV-Vis spectroscopy showed an absorption band at 340.8nm; the size of the nanoparticles is around 723 nanometer according to particle size analysis. FTIR analysis showed that functional group of synthesized silver nanoparticles belongs to free amino or carboxyl group. FTIR analysis showed the functional group of synthesized silver nanoparticles and the absorption bands were seen at 518.82cm-1, 730.01cm-1, 873.69cm-1, 1093.56cm-1, 1139.85cm-1, 1640.35cm-1, 2088.76cm-1, 3446.56cm-1. The effect of leaf extract of S. grandiflora on the survival and development of fourth instar larvae of Ae. Aegypti was sudied. LC50 values of aqueous leaf extract of S. grandiflora against Ae. aegypti and silver nanoparticles synthesized from aqueous leaf extract of S. grandiflora were 293.87 ppm and 5.548ppm respectively. The killing effect increased along with the increasing concentration of aqueous and silver nanoparticles synthesized from leaf extract of S. grandiflora. The synthesized silver nanoparticles from the leaf extract S. grandiflora were more efficient than the aqueous leaf extract. Thus the present study also suggested that the S. grandiflora leaf mediated silver nanoparticles could be used as an effective larvicidal and pupicidal agents.