Abstract
Ecbolin A and ecbolin B were isolated from ethyl acetate extract of Ecbolium viride (Forsk.) Alston root and evaluated for larvicidal and growth disturbance activities against Aedes aegypti L. (Diptera: Culicidae). For larvicidal activity, the third instar larvae of A. aegypti were exposed to different concentrations viz., 1.0, 2.5, 5.0 and 10ppm for each compound. Among the two compounds screened, ecbolin B recorded highest larvicidal activity with LC50 and LC90 values of 0.70 and 1.42ppm, respectively. In control, the larval behaviour was normal. The active compound ecbolin B was tested for growth disruption activity at sub lethal concentrations viz., 0.5, 1.0ppm and observed for malformation like larval gut elongation, larval longevity, intermediates, malformed adults, failed adult emergence and compared with methoprene. The results showed significant level of larva–pupa intermediates, pupa–adult intermediates, malformed adult emergence and less adult formation against A. aegypti. The histopathological results revealed a severe damage on the midgut epithelial columnar cells (CC) and cuboidal cells (CU) in ecbolin B treated larvae of A. aegypti. Similarly peritrophic membrane (pM) was also observed to be damaged in the treated larvae. The present results suggest that, ecbolin B could be used as a larvicidal agent against dengue vector A. aegypti.
Highlights
Mosquitoes are arthropod vectors responsible for transmitting various pathogens and mosquitoes are called as ‘Public Enemy Number One’ (WHO, 1996a)
We have reported the larvicidal and pupicidal activities of ecbolin A and ecbolin B isolated from the ethyl acetate extract of E. viride root against Culex quinquefasciatus (Cecilia et al, 2014)
A. aegypti larvae were less susceptible to ecbolin A in the present assessment and the LC50 and LC90 values of ecbolin A on larval mortality were 8.56, 17.47 ppm against the third instar larvae of A. aegypti, respectively (Table 1)
Summary
Mosquitoes are arthropod vectors responsible for transmitting various pathogens and mosquitoes are called as ‘Public Enemy Number One’ (WHO, 1996a). Aedes aegypti L. is the primary vector, which is involved in the transmission of arboviruses responsible for major diseases like dengue, dengue hemorrhagic fever, chikungunya and zika (Harrington et al, 2005; Kannathasan et al, 2011). Dengue fever is endemic to many tropical countries including India (Ahmed and Akram, 2005; Valenca et al, 2013). Mosquito borne diseases are major threat to human health. Biological and chemical compounds like Bacillus thuringiensis israelensis (Bti), Bacillus sphaericus (BS), pyrethroids, pyriproxyfen, permethrin, diflubenzuran and methoprene are used as larvicidal and growth regulating products in integrated vector management (IVM) against A. aegypti (Bellini et al., A.D. Reegan et al / Parasite Epidemiology and Control 1 (2016) 78–84
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
