Biodiversity of lactic acid bacteria of wild honey bee and Sumbawa horse milk by using RAPD-PCR

Abstract

Wild honey bee and Sumbawa horse milk is the local product from West Nusa Tenggara, Indonesia, which has the characteristic of little sour in taste. The sour taste indicates lactic acid bacteria (LAB) existence that has a potential function as probiotics. The objection of this study is analysis of lactic acid bacteria biodiversity from wild honey bee and Sumbawa horse milk by using Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR). The genome of cultured samples from M17 and MRS medium were extracted, then identified by RAPD-PCR with primer (GTG)5 (5-GTGGTGGTGGTGGTG-3). The scoring of DNA fragments was analyzed using the Numerical Taxonomy and Multivariate Analysis System (NTSYS) application. The result show five clusters from a common ancestor indicated the broad diversity of LAB in wild honey bee and Sumbawa horse milk.